Fig. 4: Detection of viable fungi increases precision for disease risk prediction. | Nature Communications

Fig. 4: Detection of viable fungi increases precision for disease risk prediction.

From: Precise in-field molecular diagnostics of crop diseases by smartphone-based mutation-resolved pathogenic RNA analysis

Fig. 4

a Scheme for the inoculation of wheat with an equal quantity of Pst mixtures containing 0%, 0.1%, 1%, 10% and 100% viable fungi, and symptom observation of infected wheat leaves after 14-day infection. b, c, Detection of Pst in samples in (a) using the colorimetric assay (b) and qPCR (c). Measurement of Pst in 14-day-infected wheat leaves using qPCR (d) and the colorimetric assay (e). Paper design; upper in (e), photographs of origami papers. Ct100 means the Ct value of Pst mixtures containing 100% viable fungi (in c) or the Ct value of wheat samples infected with Pst mixtures containing 100% viable fungi (e). Data in (b–d) are means ± SD (n = 3). Data in (e) are means (n = 6). P values from Welch’s two-sided unpaired t test in (b, c and e): **P < 0.01, ***P < 0.001, ****P < 0.0001. Source data are provided as a Source Data file.

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