Fig. 1: AVL controls aBoc through peptidergic signaling to hmc. | Nature Communications

Fig. 1: AVL controls aBoc through peptidergic signaling to hmc.

From: The head mesodermal cell couples FMRFamide neuropeptide signaling with rhythmic muscle contraction in C. elegans

Fig. 1

a Schematic showing the circuit controlling aBoc identified in this study. b Quantification of the number of aBocs per defecation cycle in adult animals of the indicated genotypes. “intestinal nlp-40” denotes nlp-40 cDNA expressed under the intestine-specific ges-1 promoter. “AVL ICE”, “AVL aex-2”, and “AVL TeTx” denote ICE, aex-2 cDNA or TeTx expressed in AVL using the nmur-3(1 kb) promoter. n = 6, 3, 3, 3, 6, 5, 3 independent animals. c Quantification of the number of aBocs per defecation cycle in adult animals of the indicated genotypes. flp-22(vj229) and frpr-17(vj265) null mutants were analyzed. “GABAergic flp-22” and “cholinergic flp-22” denote flp-22 cDNA expressed under the GABAergic-specific unc-47 and cholinergic-specific unc-129 promoter, respectively. “AVL flp-22” denotes flp-22 cDNA expressed in AVL using the nmur-3(1 kb) promoter. “neuronal frpr-17” denotes frpr-17 cDNA expressed under the pan-neuronal rab-3 promoter. “muscle frpr-17” denotes frpr-17 cDNA expressed under the muscle-specific myo-3 promoter. “hmc frpr-17” denotes frpr-17 cDNA expressed in hmc using the nmur-3(Δ) promoter. n = 6, 6, 4, 3, 8, 5, 4, 6, 3, 5 independent animals. d Left: schematic of the location of AVL and hmc in the neck region and representative images showing GFP transcriptional reporters in wild-type and hlh-8 mutants, scale bar, 40 μm. “AVL + hmc GFP” denotes expressing GFP under the numr-3(3 kb) promoter. GFP fluorescence is not detectable in hlh-8 mutants (0 out of 20 animals exhibited GFP fluorescence in hmc). Right: quantification of the number of aBocs per defecation cycle in adult animals of the indicated genotypes. “hmc ICE” and “hmc miniSOG” denote transgenes expressing ICE or miniSOG in hmc under control of the nmur-3(Δ) promoter. n = 6, 3, 5, 8, 5, 3, 11 independent animals. Data are presented as mean values ± SEM. ***P < 0.001, **P < 0.0,1 and *P < 0.05 in one-way ANOVA with Bonferroni’s correction for multiple comparisons; n.s. not significant.

Back to article page