Fig. 1: Single-cell transcriptomic atlas of human MSCs reconstructs a progressive cellular senescence process. | Nature Communications

Fig. 1: Single-cell transcriptomic atlas of human MSCs reconstructs a progressive cellular senescence process.

From: Multi-omics analysis of human mesenchymal stem cells shows cell aging that alters immunomodulatory activity through the downregulation of PD-L1

Fig. 1: Single-cell transcriptomic atlas of human MSCs reconstructs a progressive cellular senescence process.The alternative text for this image may have been generated using AI.

a Schematic representation of the study design and experimental procedure. b UMAP showing seven subpopulations of MSCs from adipose tissue, bone marrow, placenta membrane and umbilical cord. MSCs were projected together by UMAP (top) and displayed separately by tissue origin (bottom). c Heatmap displaying differences in pathway activity calculated by GSVA in distinct cell subclusters. d PAGA analysis of each MSC cluster. Nodes represent subsets, and thicker edges indicate stronger connectedness between subsets. e–g Violin plots showing average expression of cellular senescence genes, DNA repair genes and proliferation genes for each cluster. Box plot within each violin plot indicate median values, and the 25th to 75th percentiles. Asterisks on specific group represent there were statistical differences compared with cluster C1, C2 and C3. The p values were generated by two-sided one-way ANOVA with Tukey’s multiple comparisons test. (***p < 2.2 × 10−16, n = 45,955 biologically independent cells) h Dot plots showing the expression values of representative senescence-related genes for each cluster. Color represents the scaled expression values from Seurat RNA assay. The displayed values were non-batch corrected.

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