Table 1 Data collection and refinement statistics of crystal structures of RRM1-ZnF1 apo and the RRM1-ZnF1s/RNA complex
From: Structural basis for specific RNA recognition by the alternative splicing factor RBM5
Dataset | RRM1-ZnF1 | RRM1-ZnF1S + GGCU_10 |
|---|---|---|
PDB ID: 7PCV | PDB ID: 7PDV | |
Wavelength (Å) | 0.87 | 1.28 |
Resolution range (Å) | 32.1–2.42 (2.5–2.4)a | 45.1–3.5 (3.6–3.5) |
Space group | C 1 2 1 | P 1 21 1 |
Unit cell (Å) | 60.38 39.88 96.85 90 96.031 90 | 51.23 65.89 94.91 90 90.046 90 |
Total reflections | 26,714 (2103) | 25,683 (4171) |
Unique reflections | 8711 (751) | 7687 (774) |
Multiplicity | 3.1 (2.8) | 1.8 (1.83) |
Completeness (%) | 97.6 (85.8) | 94.1 (94.4) |
Mean I/sigma(I) | 5.60 (0.8) | 1.98 (1.0) |
Wilson B-factor | 52.36 | 53.96 |
R-merge | 0.15 (1.20) | 0.36 (1.11) |
R-meas | 0.18 (1.48) | 0.41 (1.13) |
R-pim | 0.10 (0.84) | 0.23 (0.69) |
CC1/2 | 0.99 (0.18) | 0.93 (0.52) |
Reflections used in refinement | 8707 (751) | 7687 (774) |
Reflections used for R-free | 436 (38) | 537 (57) |
R-work | 0.26 (0.39) | 0.34 (0.32) |
R-free | 0.31 (0.40) | 0.39 (0.43) |
Number of non-hydrogen atoms | 1889 | 3426 |
Macromolecules | 1850 | 3422 |
Ligands | 2 | 4 |
Solvent | 37 | 0 |
Protein residues | 232 | 428 |
RMS(bonds) (Å) | 0.003 | 0.002 |
RMS (angles) (°) | 0.69 | 0.61 |
Ramachandran favored (%) | 97.37 | 87.62 |
Ramachandran allowed (%) | 2.19 | 12.14 |
Ramachandran outliers (%) | 0.44 | 0.24 |
Rotamer outliers (%) | 2.6 | 0.00 |
Clashscore | 11.62 | 10.9 |
Average B-factor | 58.16 | 31.47 |
Macromolecules | 58.31 | 31.52 |
Ligands | 46.86 | 25.04 |
Solvent | 51.44 | – |
Coordinate error (Å, Luzzati plot) | 0.44 | 1.0 |
