Table 3 Isothermal titration calorimetry data for RBM5 RNA binding domains
From: Structural basis for specific RNA recognition by the alternative splicing factor RBM5
Protein | RNA | Av. KDa (μM) | KDb (μM) | ΔHb (kcal/mol) | −TΔSb (kcal/mol) | Binding stoichio-metry (N)b |
|---|---|---|---|---|---|---|
RRM1 | CU_9 | 19.7 ± 6.5 | 13.2 ± 3.1 26.2 ± 6.4 | −7.3 ± 0.8 −10.0 ± 1.7 | 0.61 3.76 | 1.04 1 (fixed) |
RRM1 | GGCU_12 | 1.88 ± 0.95 | 0.93 ± 0.18 2.83 ± 0.47 | −11.7 ± 0.4 −10.6 ± 0.4 | 3.49 3.06 | 0.97 1.01 |
RRM2 | GGCU_12 | 172 ± 6 | 171 ± 8 174 ± 8 | −24.5 ± 0.7 −25.8 ± 0.8 | 19.2 20.6 | 1 (fixed) 1 (fixed) |
RRM1-ZnF1 wt | CU_9 | 5.56 ± 2.83 | 8.39 ± 0.87 2.73 ± 0.37 | −11.8 ± 1.1 −6.23 ± 0.27 | 4.88 1.36 | 1.02 0.94 |
RRM1-ZnF1 wt | GGCU_12 | 0.153 ± 0.069 | 0.084 ± 0.012 0.222 ± 0.040 | −16.2 ± 0.2 −14.1 ± 0.3 | 6.57 4.99 | 1.02 0.81 |
RRM1-ZnF1 F142/144A | GGCU_12 | 0.448 ± 0.036 | 0.469 ± 0.055 0.427 ± 0.047 | −12.7 ± 0.2 −12.0 ± 0.2 | 4.05 3.31 | 0.7 0.97 |
RRM1-ZnF1 F202A | GGCU_12 | 0.379 ± 0.041 | 0.368 ± 0.046 0.390 ± 0.067 | −9.58 ± 0.20 −9.74 ± 0.27 | 0.80 1.00 | 1.03 1.01 |
RRM1-ZnF1 R198E | GGCU_12 | 2.66 ± 0.38 | 2.69 ± 0.27 2.63 ± 0.70 | −10.7 ± 0.3 −8.37 ± 0.83 | 3.08 0.75 | 1.05 1.11 |
RRM1-ZnF1S | GGCU_12 | 0.263 ± 0.027 | 0.273 ± 0.036 0.253 ± 0.041 | −10.5 ± 0.2 −10.9 ± 0.2 | 1.56 1.94 | 1.08 1.06 |
RRM1-ZnF1S | eGGCU_12 | 0.641 ± 0.125 | 0.766 ± 0.116 0.516 ± 0.077 | −15.2 ± 0.4 −14.2 ± 0.3 | 6.90 5.66 | 0.63 0.60 |
RRM1-ZnF1S-RRM2 | GGCU_12 | 0.133 ± 0.012 | 0.127 ± 0.007 0.138 ± 0.022 | −19.1 ± 0.1 −18.1 ± 0.4 | 9.68 8.70 | 0.98 1.01 |
