Table 2 Kinetic parameters describing PfCRTDd2-, E207A-, and E207H-mediated CQ transport at pH 4.5 and pH 6.0

From: pH-dependence of the Plasmodium falciparum chloroquine resistance transporter is linked to the transport cycle

PfCRT variant

 

Vmax ± SEM

KM ± SEM

p value

  

pmol h−1 oocyte −1

μM

 

PfCRTDd2

pH 6.0

32 ± 2

270 ± 50

1.1 × 10–10 (df = 12, F = 182.27)

PfCRTDd2

pH 4.5

14 ± 2

200 ± 60

 

  E207A

pH 6.0

11 ± 1

150 ± 30

0.15 (df = 12, F = 2.26)

  E207A

pH 4.5

11 ± 1

180 ± 30

 

  E207H

pH 6.0

29 ± 1

230 ± 20

1.4 × 10–5 (df = 12, F = 32.72)

  E207H

pH 4.5

13 ± 1

230 ± 35

 
  1. Statistical significance was determined between the CQ transport activity at pH 4.5 and 6.0, using a two-tailed F-test.
  2. Vmax maximal CQ transport velocity, KM Michaelis–Menten constant, SEM standard error of the mean, df degrees of freedom, F F-factor.
Back to article page