Fig. 7: Restoration of PITPNA in isolated islets of T2D human subjects rescues pancreatic beta-cell function.
From: Restoration of PITPNA in Type 2 diabetic human islets reverses pancreatic beta-cell dysfunction
a Western blot analysis of PITPNA in human non-diabetic (ND), and T2D islets after either lentiviral-mediated over-expression of PITPNA (T2D-PITPNA OE) or treatment with a control lentivirus (T2D) (n = 2). b Quantification of insulin release from isolated islets from T2D donors after either lentiviral-mediated over-expression of PITPNA (T2D-PITPNA OE) or treatment with a control lentivirus (T2D) (n = 4). P0 = 0.0284, P5 = 0.7128, P15 = 0.0363. c, d Quantification of GOLGIN97 and GOLPH3 colocalization after immunostaining of isolated human islets from T2D donors after either lentiviral-mediated over-expression of PITPNA (T2D-PITPNA OE) or treatment with a control lentivirus (T2D) (n = 15). P < 0.0001. e Representative transmission electron micrographs of pancreatic beta-cells of non-diabetic (ND) or T2D human donors after treatment with a control lentivirus (T2D) or lentiviral-mediated over-expression of PITPNA (T2D-PITPNA OE). The second raw image represents a zoom-in view of the white box from the first raw image. Immature secretory granules (blue box), mature secretory granules (red box), crystal-containing granules (yellow box), and empty secretory granules (orange box). Blue arrows identify docked granules. f Quantification of granule density, g docked vesicles, and h granule profile: immature secretory granule (ISG), mature secretory granules (MSG), crystal-containing granules (CCG), and empty secretory granules (ESG) in beta-cells of non-diabetic (ND) or T2D human donors after treatment with a control lentivirus (T2D) or lentiviral-mediated over-expression of PITPNA (T2D-PITPNA OE) (n = 4). Data are presented as mean values ± SEM for (b), (c), (f), (g), (h). *P < 0.05, **P < 0.01, ***P < 0.001. Ordinary one-way ANOVA with Turkey’s multiple comparisons test was used for (f), (g), (h). Two-tailed unpaired Student t-test were used in for (b), (c). All primary source data are reported in the Source data file.
