Fig. 1: PKM2 expression increases in activated neutrophils. | Nature Communications

Fig. 1: PKM2 expression increases in activated neutrophils.

From: The metabolic function of pyruvate kinase M2 regulates reactive oxygen species production and microbial killing by neutrophils

Fig. 1

a Pkm2 expression analysis in myeloid cells from the Immunological Genome Project (ImmGen) bulk-population RNA-seq database14. b Immunoblot analysis of PKM2 expression in BM-isolated neutrophils from wild-type (Pkm2fl/fl) and PKM2-deficient (Pkm2∆Lyz2) mice. Cells were activated with Zy/op (100 µg/mL) or medium only (Ctrl) for 6 h. β-actin was used as the loading control. Representative of three independent experiments. c Immunoblot analysis of PKM2 conformational states in murine neutrophils after activation with Zy/op for 6 h using DSS cross-linking assay. Representative of two independent experiments. d Confocal immunofluorescence analysis of Zy/op-activated murine neutrophils stained for PKM2 (green) and DAPI (blue). The scale bar indicates 5 μm. Representative of three independent experiments. e Immunoblot analysis of PKM2 expression in blood-isolated neutrophils from three healthy volunteers. Cells were activated with Zy/op for 6 h. β-actin was used as the loading control. Representative of three independent experiments. f Confocal immunofluorescence analysis of Zy/op-activated human neutrophils stained for PKM2 (green) and DAPI (blue). The scale bar indicates 5 μm. Representative of three independent experiments. Clip art provided by Biorender. Source data are provided in the Source data file.

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