Fig. 4: Metabolic activity of PKM2 regulates ROS production in neutrophils.

a GLUT1 expression in wild-type (Pkm2^fl/fl) and PKM2-deficient (Pkm2^∆Lyz2) activated with Zy/op (100 µg/mL) for 1 h. Representative histogram and graph bar showing the GLUT1 frequency and median fluorescence intensity (MFI). Gating strategies are shown in Supplementary Fig. 5a. n = 6 mice per group in two independent experiments. b Glucose uptake in wild-type and PKM2-deficient neutrophils activated with Zy/op for 30 min. Representative histogram and graph bar showing frequency and MFI for 2-NBDG. Gating strategies are shown in Supplementary Fig. 5a. n = 6 mice per group in two independent experiments. c Pyruvate Kinase (PK) activity in neutrophils activated with Zy/op for 1 h. n = 5 mice per group in two independent experiments. d Pyruvate production by neutrophils activated with Zy/op for 1 h. n = 4 mice per group in two independent experiments. e Lactate production by wild-type and PKM2-deficient mouse neutrophils and by human neutrophils pre-treated with oxalate (3 mM) for 1 h and activated with Zy/op for 1 h. n = 5 mice per group in two independent experiments and n = 5 donors in five independent experiments. f Glycolytic metabolites from ¹³C-glucose tracing in wild-type and PKM2-deficient neutrophils activated with Zy/op for 1 h. The y-axis refers to the height of the chromatography peak obtained by LC-MS. n = 3 mice per group in three independent experiments. Error bars are mean ± SEM. p values were determined by one-way ANOVA followed by Tukey’s post hoc test. Clip art provided by Biorender. Source data are provided in the Source data file.