Fig. 2: Translation of IGG-mediated bicistronic cassettes.
From: A polycistronic system for multiplexed and precalibrated expression of multigene pathways in fungi
a Translation re-initiation at the distal gene GFP, mediated by IGG6. Translation of Tdh3p and GFP from different gene expression units was monitored by Western blotting and fluorescence microscopy, respectively. The scale bars for the images are 20 μm. Red triangles represent a translation blocking sequence (TBS). Uncropped Western blots are available (Supplementary Fig. 9). b Model for the role of IGG during bicistronic expression in eukaryotes. Two contiguous genes linked by IGG are co-transcribed into a bicistronic mRNA under the control of the same promoter. The mRNA is then translated into two individual proteins by the ribosome which is not released after translation of the upstream open reading frame (ORF), but rather resumes scanning and initiates the translation of the downstream ORF.
