Fig. 2: Booster dose of NVX-CoV2373 enhanced neutralizing antibody responses against BA.5 in hamsters primarily vaccinated with Ad26.COV2.S or BNT162b2. | Nature Communications

Fig. 2: Booster dose of NVX-CoV2373 enhanced neutralizing antibody responses against BA.5 in hamsters primarily vaccinated with Ad26.COV2.S or BNT162b2.

From: Immunogenicity and efficacy of vaccine boosters against SARS-CoV-2 Omicron subvariant BA.5 in male Syrian hamsters

Fig. 2

Four-to-five-week-old male hamsters were immunized either with one dose (1010 or 108 vp) of Ad26.COV2.S or two doses of BNT162b2 (5 μg per dose). After 6 months (day 168), animals were boosted with heterologous vaccines: 0.5 µg mRNA-1273 (n = 12 per group) or 1 μg rS/15 μg Matrix-M NVX-CoV2373 (n = 12 per group), or with the homologous vaccines: 108 vp Ad26.COV2.S (n = 12 per group) or 0.5 μg BNT162b2 (n = 12). A sham-boosted group (PBS) was included for comparison (n = 13 for 108 vp of Ad26.COV2.S or n = 14 for 1010 vp of Ad26.COV2.S and BNT162b2 groups). Blood was collected immediately prior to boosting as well as at 1 and 3 months post-boost (days 196 and 252). Neutralizing antibody titers against Omicron BA.5 were determined by foci reduction neutralization assay (FRNT), with the FRNT50 values defined as the maximum serum dilution to neutralize at least 50% of infectious virus. a–c Paired analysis of pre-boost, 1 month post-boost, and 3 months post-boost serum neutralizing titers against Omicron BA.5 from hamsters that received as primary immunizations (a) 1010 vp Ad26.COV2.S, (b) 108 vp Ad26.COV2.S, or (c) two 5 μg doses of BNT162b2. d–f Grouped analysis of serum neutralizing antibody responses of samples collected pre-boost, 1 month post-boost and 3 months post-boost against BA.5 in hamsters that received as primary immunizations (d) 1010 vp Ad26.COV2.S, (e) 108 vp Ad26.COV2.S, or (f) two 5 μg doses of BNT162b2. Colors designate experimental cohorts, data points represent individual subjects, bar heights represent the geometric mean titer (GMT), error bars represent the 95% confidence interval, and dotted lines represent the lower limit of detection. GMTs and fold-change values are noted above the respective cohorts. Groups were compared by non-parametric ANOVA (Friedman’s test) with Dunn’s multiple comparison post-test (ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). All panels were generated using GrapPad Prism v9.4 software. See Source Data for complete data.

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