Fig. 4: pH-dependent sub-state conductance in in hKir2.1[G177E].
From: Subunit gating resulting from individual protonation events in Kir2 channels
A Representative traces of Kir2.1[G177E] channel activity at different pH. All-point histograms are presented on the right of each trace. B Expanded interval of the trace from (A) reveals a closed and four conducting states at pH 7.4. C Representative traces of WT Kir2.1 channel activity reveal only a single open state at pH 6.8 or 7.4. D Sub-states amplitudes of cKir2.1[G177E] mutant at −120 mV membrane at different pH. Inset: normalized conductances of individual hKir2.1[G177E] mutant sub-states at different pH. All measurements were made on single channels in symmetric 150 mM KCl buffer with indicated pH. Data in D shown as mean ± s.e.m. (n = 100–1000 transitions within each patch, N = 2 patches, for each conductance level).
