Fig. 5: Comparison of the polymerase of the six species of crAssBcn phages.

a. hylogenetic tree constructed with the polA of crAssBcn phage, other PolA and PolB proteins available in databases and reported in crass-like phages by Yutin et al., (2021). b Detail of the branch of the phylogenetic tree were crAssBcn phages are located showing the two separated branches containing the 706aa and the 775aa family A polymerases. c Multiallignment of PolA of the six species of crAssBcn phages. Upper part shows the polymerase A domains using the polymerase A gene of phage ΦCrAss001 as reference. From top to bottom this chart shows first the length of the protein. Small triangles indicate the aminoacids involved in conserved active, catalytic and DNA binding sites of each domain. Colored bars show the closest hits found in the conserved domain database (CCD) for each domain, these can be specific hits (with a high confident association) or non-specific and the superfamily to which the highest-ranking hit belongs. In the bottom part, the alignment of the polymerases of the six species (groups I-VI) and ΦCrAss001. Vertical red shadows bands highlight the gaps observed between the 775aa and the 706aa polA.