Fig. 2: Novel mouse models CaMKIIαCre/T29 (transgenic) and Kcng4Cre (knock-in/knock-out) to discriminate PC subpopulations.
From: Purkinje cell microzones mediate distinct kinematics of a single movement
A–C and E,F Serial transverse sections of the cerebellum of CaMKIIαCre/T29;Ai14 (A) and Kcng4Cre;Ai14 (E) mouse immunolabeled for Hsp25 (B), KCTD12 (C), and AldoC (F). White brackets indicate the Hsp25 domains (B) and the corresponding TdTomato+ domains in the CaMKIIαCre/T29;Ai14 (A), as well as KCTD12+ domains (C). Yellow brackets indicate the TdTomato+ domains from the CaMKIIαCre/T29;Ai14, which do not correspond to any Hsp25+ domains, with a zoom on the flocculus (A5 and B5) to highlight that in this particular region, CaMKIIαCre/T29 expression extends beyond Hsp25 and matches KCTD12 expression (expression pattern confirmed in n = 4 CaMKIIαCre/T29;Ai14 mice). Blue and red brackets and dotted lines show the complementation of TdTomato +, from the Kcng4Cre;Ai14 and the AldoC+ populations (E1 and F1). Scale bar = 1 mm (applies to all images). D Schematic representation of Cre expression in the CaMKIIαCre/T29 relative to Hsp25 and KCTD12 expression profiles. G Schematic representation of Cre expression in the Kcng4Cre relative to AldoC expression profile.
