Fig. 3: Characterization of SMNDC1’s interactome by proximity labeling.
From: Pharmacological perturbation of the phase-separating protein SMNDC1
a Scheme of proximity labeling by APEX2 fusion proteins followed by mass spectrometry-based proteomics. b Immunofluorescence images (αTC1 WT) with staining against SMNDC1 (green), Biotin via Streptavidin (red) and nuclear staining DAPI (blue). c Volcano plot showing log2 abundance against -log10 adjusted p-value (one-way ANOVA, Benjamini-Hochberg correction for multiple comparisons) of APEX2-SMNDC1FL versus APEX2-SMNDC1TD biotinylated and enriched proteins. Highlighted dots in green indicate 750 enriched proteins (adjusted p-value < 0.1, abundance ratio > 1.1). d Enrichr analysis of APEX2-SMNDC1FL enriched proteins, GO Biological Process 2021 terms plotted with their odds ratio and their adjusted p-value (Benjamini–Hochberg method for correction for multiple hypotheses testing). Terms with a -log10 adjusted p-value >60 colored green. e Venn diagram showing the overlap of proteins identified by SMNDC1-CoIP (light blue), and APEX2-SMNDC1FL enriched (green). f Venn diagram showing the overlap of proteins identified by SRSF7-APEX2 (light blue), and APEX2-SMNDC1FL enriched (green).
