Fig. 4: Inflammation-Dependent Alternative (IDA) macrophages are widely distributed in ulcerative colitis and present in Crohn’s disease granulomas.
a CosMxTM SMI distribution of IDA and M1 macrophages in IBD colonic samples. Images from 1 representative UC (2 Fields of View (FoV)) and 1 CD (4 FoVs) patients (out of a total of 6 patients analyzed) are shown. Scale bar = 200 μm. b CD colonic sample with multiple granulomas (CD b patient). (i) Two FoVs from this patient are indicated by squares and other granulomas found in the same sample by yellow arrows. (ii) Macrophages within granulomas are shown by CosMxTM SMI in FoVs 4 and 5 and protein expression (Scale bar = 200 μm) of (iii) CD68 and (iv) CD209 is shown by immunohistochemistry on the same tissue sections (Scale bar = 100 µm). c Double NRG1 in situ hybridization and CD68 or CD209 immunostaining in tissue sections from the CD patient (CD b) containing abundant granulomas. In situ hybridization of NRG1 shows an increasing gradient of expression towards the apical mucosa. Granulomas are indicated by dotted circles (Scale bar = 100 µm). d Magnified pictures of representative granulomas of the same CD tissue stained for NRG1 using in situ hybridization and CD68 or CD209 immunostaining. Granulomas are indicated by dotted circles and NRG1 positive cells are shown by arrows (Scale bar = 100 µm). Source data are provided as a Source Data file. HC, healthy controls. IBD, inflammatory bowel disease. CD, Crohn’s disease. UC, ulcerative colitis.
