Fig. 3: The construction of LungMAP Mouse Lung Development CellRef.
From: Guided construction of single cell reference for human and mouse lung
A The developmental time points of mouse lung single-cell transcriptome data used for the guided CellRef construction. B Uniform manifold approximation and projection (UMAP) visualization of the seed cells representing 40 cell types of the developing mouse lung, termed LungMAP Mouse Lung Development CellRef Seed. Cells were colored by predicted seed identities. C UMAP visualization of CellRef for normal mouse lung development, named LungMAP Mouse Lung Development CellRef. Cells were colored by their predicted identities. D Validation of seed cell identities using expression of cell type selective marker genes. E Lineage relationships among mouse lung cell types were reconstructed using hierarchical clustering analysis using pseudo-bulk gene expression profiles. Color represents Pearson’s correlation value of pseudo-bulk expression profiles. Labels ending with “.Seed” represent pseudo-bulk profiles created by averaging gene expression in the cells of each cell type in the mouse lung CellRef Seed, while labels ending with “.CellRef” represent pseudo-bulk profiles created using gene expression of each cell type in the complete mouse lung CellRef.
