Fig. 3: Loss-of-function of ORF3 eliminates Se-mediated HMS and segregation distortion. | Nature Communications

Fig. 3: Loss-of-function of ORF3 eliminates Se-mediated HMS and segregation distortion.

From: Two complementary genes in a presence-absence variation contribute to indica-japonica reproductive isolation in rice

Fig. 3: Loss-of-function of ORF3 eliminates Se-mediated HMS and segregation distortion.The alternative text for this image may have been generated using AI.

a Position of two editing targets in ORF3. PAM sequence is highlighted in red. b F1 hybrids with three orf3 alleles exhibited normal pollen fertilities. Pollen fertility is shown as means ± SD (n = 10 biologically independent samples). Two-tailed Student’s t tests were performed to compare the pollen fertility of orf3/T65 F1 hybrids and E9/T65 F1 hybrids (***p < 0.001). Scale bars = 50 μm. c Segregation of pollen fertility in F2 progeny derived from E9/T65 or orf3/T65 crosses. T/T, E/E and T/E represent the homozygous genotype for T65 and E9 and the heterozygous genotype, respectively. Source data are provided as a Source Data file.

Back to article page