Fig. 4: ORF4 rescues T65-type pollen in a gametophytic manner. | Nature Communications

Fig. 4: ORF4 rescues T65-type pollen in a gametophytic manner.

From: Two complementary genes in a presence-absence variation contribute to indica-japonica reproductive isolation in rice

Fig. 4

a ORF4 hemizygous transgenic T0 plants were crossed with E9 to generate F1 plants without the ORF4 transgene (T/E; -/-) (left, ~50% pollen fertility) and F1 plants with the ORF4 transgene (T/E; ORF4/-) (right, ~75% pollen fertility). Scale bars = 100 μm. b Comparison of pollen fertility between F1 plants with (T/E; ORF4/-) and without (T/E; -/-) the ORF4 transgene. Pollen fertility is shown as means ± SD (n = 6 biologically independent samples). c Comparison of ORF4 expression between F1 plants with (T/E; ORF4/-) and without (T/E; -/-) the ORF4 transgene. Data are presented as means ± SD (n = 3 biologically independent experiments). d A schematic showing expected genotypes in F2 progeny from selfing of F1 plants with a single hemizygous copy of the ORF4 transgene (T/E; ORF4/-). e Observed genotype frequencies and pollen fertility of F2 progeny from a selfed F1 plant (T/E; ORF4/-). The observed (N) and expected (NE) numbers of individual genotypes among 240 F2 progeny are shown. Pollen fertility is shown as means ± SD (n = 10 biologically independent samples). Significant differences were determined by two-tailed Student’s t tests (***p < 0.001). Source data are provided as a Source Data file.

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