Fig. 3: Immune responses activated by LN-TCV with NIR laser irradiation at post-injection day 9 in the lymph node. | Nature Communications

Fig. 3: Immune responses activated by LN-TCV with NIR laser irradiation at post-injection day 9 in the lymph node.

From: Generation of whole tumor cell vaccine for on-demand manipulation of immune responses against cancer under near-infrared laser irradiation

Fig. 3: Immune responses activated by LN-TCV with NIR laser irradiation at post-injection day 9 in the lymph node.The alternative text for this image may have been generated using AI.

a PA images (left) and corresponding PA signal quantitative analysis (right) around the inguinal lymph nodes of mice with different treatments (PBS, LN-TCV, LN-TCV + L, and LN-TCV + 2L) for showing the homing of DCs. The P values of LN-TCV to PBS, LN-TCV + L to LN-TCV, LN-TCV + 2L to LN-TCV + L, and LN-TCV + 2L to LN-TCV were <0.0001, 0.0004, 0.0002, and <0.0001, respectively. b Representative flow cytometry plots (left) and quantitative analysis (right) of mature DCs (CD80+CD86+ in CD11c+ gate) in the lymph nodes of mice with indicated treatments. The P values of LN-TCV to PBS, LN-TCV + L to LN-TCV, LN-TCV + 2L to LN-TCV + L, and LN-TCV + 2L to LN-TCV were 0.0004, 0.0003, 0.0015, and <0.0001, respectively. c Immunofluorescence analysis (left) and quantitative analysis (right) of CD8+ T cells in the lymph nodes of mice with indicated treatments. Red: CD8+T cells; Blue: cell nucleus. The images were presented with the same magnification. The P values of LN-TCV to PBS, LN-TCV + L to LN-TCV, LN-TCV + 2L to LN-TCV + L, and LN-TCV + 2L to LN-TCV were <0.0001, 0.0003, 0.0002, and <0.0001, respectively. d Representative flow cytometry plots (left) and quantitative analysis (right) of IFN-γ-producing CD8+ T cells in the lymph nodes of mice with indicated treatments. The P values of LN-TCV to PBS, LN-TCV + L to LN-TCV, LN-TCV + 2L to LN-TCV + L, and LN-TCV + 2L to LN-TCV were 0.0043, 0.0097, 0.0006, and <0.0001, respectively. e Representative flow cytometry plots (left) and quantitative analysis (right) of granzyme B-producing CD8+ T cells in the lymph nodes of mice with indicated treatments. The P values of LN-TCV to PBS, LN-TCV + L to LN-TCV, LN-TCV + 2L to LN-TCV + L, and LN-TCV + 2L to LN-TCV were 0.0010, 0.0020, 0.0002, and <0.0001, respectively. f Clone frequencies of CDR3 sequences in the T cells derived from the lymph nodes of mice with indicated treatments (n = 1 mice per group). Quantitative data in (a–e) were represented as mean values ± s.d., n = 3 mice per group. P values in (a–e) were calculated by one-way ANOVA. The images and flow cytometry plots in (a–e) were representative of three mice. Source data were provided in the Source data file.

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