Fig. 2: Hematopoietic Card9 deficiency accelerates atherosclerosis in Ldlr^-/- mice.

A Card9 mRNA expression by peritoneal macrophages isolated from chimeric Ldlr^-/-Card9^+/+ and Ldlr^-/-Card9^-/- mice (n = 5/group). B body weight at sacrifice after 8 weeks of fat diet (n = 14/group). C plasma cholesterol levels at sacrifice after 8 weeks of fat diet (n = 14/group). D representative photomicrographs and quantitative analysis of atherosclerotic lesions on the thoraco-abdominal aortas from chimeric female Ldlr^-/-Card9^+/+ and Ldlr^-/-Card9^-/- mice after 8 weeks of fat diet (n = 7 Ldlr^-/-Card9^+/+ and n = 6 Ldlr^-/-Card9^-/-); Scale bar 1 mm. E representative photomicrographs and quantitative analysis of atherosclerotic lesions in the aortic sinus of chimeric Ldlr^-/-Card9^+/+ and Ldlr^-/-Card9^-/- mice after 8 weeks of fat diet (2 experiments pooled, n = 14/group); Scale bar 200 μm. F representative photomicrographs and quantitative analysis of macrophage accumulation (MOMA staining, red) in atherosclerotic lesions of chimeric Ldlr^-/-Card9^+/+ and Ldlr^-/-Card9^-/- mice after 8 weeks of fat diet (2 experiments pooled, n = 14/group); Scale bar 100 μm. G Representative photomicrographs and quantitative analysis of acellular area (Masson’s Trichrome) of chimeric Ldlr^-/-Card9^+/+ and Ldlr^-/-Card9^-/- mice after 8 weeks of fat diet (2 experiments pooled, n = 14/group); Scale bar 100 μm. H Cytokine production (ELISA in the supernatant) by Lps/Ifnγ-stimulated splenocytes isolated from chimeric Ldlr^-/-Card9^+/+ and Ldlr^-/-Card9^-/- mice (n = 5 Ldlr^-/-Card9^+/+ and n = 6 Ldlr^-/-Card9^-/-); Data are presented as mean values ±SD. Two-tailed Mann–Whitney test. Source data are provided as a Source Data file.