Fig. 3: Card9 deficiency accelerates atherosclerosis in immunodeficient Apoe-/-Rag2-/-mice. | Nature Communications

Fig. 3: Card9 deficiency accelerates atherosclerosis in immunodeficient Apoe-/-Rag2-/-mice.

From: Genetic inhibition of CARD9 accelerates the development of atherosclerosis in mice through CD36 dependent-defective autophagy

Fig. 3: Card9 deficiency accelerates atherosclerosis in immunodeficient Apoe-/-Rag2-/-mice.The alternative text for this image may have been generated using AI.

A Card9 mRNA expression by peritoneal macrophages isolated from male Apoe-/-Rag2-/-Card9+/+ and Apoe-/-Rag2-/-Card9-/- mice (n = 6/group). B Weight at sacrifice after 6 weeks of fat diet (3 pooled experiments, n = 16–18/group). C plasma cholesterol levels at sacrifice after 6 weeks of fat diet (3 pooled experiments, n = 14 Apoe-/-Rag2-/-Card9+/+ and n = 16 Apoe-/-Rag2-/-Card9-/-). D representative photomicrographs and quantitative analysis of atherosclerotic lesions on the thoraco-abdominal aorta of of Apoe-/-Rag2-/-Card9+/+ and Apoe-/-Rag2-/-Card9-/- mice after 6 weeks of fat diet (n = 6 Apoe-/-Rag2-/-Card9+/+ and n = 7 Apoe-/-Rag2-/-Card9-/-); E representative photomicrographs and quantitative analysis of atherosclerotic lesions in the aortic sinus of Apoe-/-Rag2-/-Card9+/+ and Apoe-/-Rag2-/-Card9-/- mice after 6 weeks of fat diet (3 experiments pooled, n = 14 Apoe-/-Rag2-/-Card9+/+ and n = 16 Apoe-/-Rag2-/-Card9-/-); Scale bar 200 μm. F representative photomicrographs and quantitative analysis of macrophage accumulation (MOMA staining, red) in atherosclerotic lesions of Apoe-/-Rag2-/-Card9+/+ and Apoe-/-Rag2-/-Card9-/- mice after 6 weeks of fat diet (3 experiments pooled, n = 14 Apoe-/-Rag2-/-Card9+/+ and n = 16 Apoe-/-Rag2-/-Card9-/-); Scale bar 100 μm. G Representative photomicrographs and quantitative analysis of acellular area (Masson’s Trichrome) of Apoe-/-Rag2-/-Card9+/+ and Apoe-/-Rag2-/-Card9-/- mice after 6 weeks of fat diet (3 experiments pooled, n = 14 Apoe-/-Rag2-/-Card9+/+ and n = 16 Apoe-/-Rag2-/-Card9-/-); Scale bar 100 μm. H Cytokine production (ELISA) by Lps/Ifnγ-stimulated splenocytes isolated from Apoe-/-Rag2-/-Card9+/+ and Apoe-/-Rag2-/-Card9-/- mice (n = 6/group). Data are presented as mean values ±SD. Two-tailed Mann–Whitney test. Source data are provided as a Source Data file.

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