Fig. 7: Pro-atherogenic effect of hematopoietic Card9 deficiency was abolished in the absence of Cd36. | Nature Communications

Fig. 7: Pro-atherogenic effect of hematopoietic Card9 deficiency was abolished in the absence of Cd36.

From: Genetic inhibition of CARD9 accelerates the development of atherosclerosis in mice through CD36 dependent-defective autophagy

Fig. 7: Pro-atherogenic effect of hematopoietic Card9 deficiency was abolished in the absence of Cd36.The alternative text for this image may have been generated using AI.

A representative blots and quantification of AMPK phosphorylation on BM-derived macrophages from Cd36-/-Card9+/+ and Cd36-/-Card9-/- macrophages after exposure to oxLDL (n = 6/group/condition). B Quantitative analysis and representative photomicrographs of P62 accumulation in macrophages from Cd36-/-Card9+/+ and Cd36-/-Card9-/- macrophages after 8-h exposure to oxLDL, Scale bar 100 μm (n = 6/group/condition). C quantification of intracellular Cholesterol of BM-derived macrophages from Cd36-/-Card9+/+ and Cd36-/-Card9-/- mice after 3 and 6 h exposure to ox-LDL (n = 5/group/timepoint). D Flow cytometry quantification of Annexin V+ apoptotic BM-derived macrophages from Cd36-/-Card9+/+ and Cd36-/-Card9-/- mice after 24-h stimulation with high concentration of oxLDL (50 μmol/l; Baseline n = 6/group, OxLDL n = 7 Cd36-/-Card9+/+ and n = 8 Cd36-/-Card9-/-). E Cytokine production by BMDMs from Cd36-/-Card9+/+ and Cd36-/-Card9-/- mice after stimulation (ELISA in the supernatant, n = 6/group). F Cd36 mRNA expression in the spleen from Ldlr-/- mice and chimeric Ldlr-/-Cd36-/-Card9+/+ and Ldlr-/-Cd36-/-Card9-/- mice (n = 20/10/10). G Card9 mRNA expression in the spleen from Ldlr-/- mice and chimeric Ldlr-/-Cd36-/-Card9+/+ and Ldlr-/-Cd36-/-Card9-/- mice (n = 20/10/10). H–K characterization of chimeric male Ldlr-/-Cd36-/-Card9+/+ (n = 10) and Ldlr-/-Cd36-/-Card9-/- mice (n = 11). H cholesterolemia in chimeric mice at sacrifice. I representative photomicrographs and quantitative analysis of atherosclerotic lesions in the aortic sinus of chimeric Ldlr-/-Cd36-/-Card9+/+ and Ldlr-/-Cd36-/-Card9-/- mice after 8 weeks of fat diet; Scale bar 200 μm. J representative photomicrographs and quantitative analysis of macrophage accumulation (MOMA staining, red) in atherosclerotic lesions of chimeric Ldlr-/-Cd36-/-Card9+/+ and Ldlr-/-Cd36-/-Card9-/- mice after 8 weeks of fat diet; Scale bar 100 μm. K Representative photomicrographs and quantitative analysis of acellular area (Masson’s Trichrome) of chimeric Ldlr-/-Cd36-/-Card9+/+ and Ldlr-/-Cd36-/-Card9-/- mice after 8 weeks of fat diet; Scale bar 100 μm. Data are presented as mean values ±SD. Two-tailed Mann–Whitney test. Source data are provided as a Source Data file.

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