Fig. 4: Alterations in histone distribution associated with the induction of dOCRs.

a–g Metagene plots (see Methods) showing the distribution of (a, d) H3, (e) H4, (b, f) H3K27me3, and (c, g) H3K36me3 for genes with strong induction of dOCRs, i.e., Cluster 5 genes from Fig. 1b, in mock and WT strain 17 infections without (a–c) and with PAA treatment (d–g). Metagene plots for H1 in mock and WT strain 17 infections with PAA treatment for Cluster 5 and corresponding metagene plots for genes without induction of dOCRs are shown in Supplementary Fig. 11a–f. The color track at the bottom of each subfigure indicates the significance of paired two-sided Wilcoxon tests comparing the normalized transcript coverages of genes for each position between mock and WT infection. P‐values are adjusted for multiple testing with the Bonferroni method within each subfigure; color code: red = adj. P-value ≤ \({10}^{-5}\); orange = adj. P-value ≤ \({10}^{-3}\); yellow: adj. P-value ≤ 0.05. h Metagene plots showing the distribution of H1 in mock, WT strain F and ΔICP22 infection for genes with strong dOCR induction, i.e., Cluster 5 genes. P-values for pairwise comparisons between mock and WT strain F infection, WT strain F and ΔICP22 infection, and mock and ΔICP22 infection, respectively, were calculated as for (a–g) and are shown in Supplementary Fig. 12b–d. Source data are provided as a Source Data file.