Fig. 3: Comparison of the aggregation propensity in light of the charge distribution of the APRs.

The total charge (z - black dotted line - right vertical axis) and the calculated relative abundance of differently charged APR microspecies as a function of the pH are displayed (a) in the presence, and (b) in the absence of an Asp/Glu acidic gatekeepers. The green background indicates the pH range where the amyloid formation was detected by CD spectroscopy.