Table 1 Reagents needed for immunofluorescence analysis
Antibody | Company | Catalog no. | Species | Dilution factor |
|---|---|---|---|---|
PfHSP70 | StressMarq Biosciences | SPC-186 | Rabbit | 1:75 |
PfCSP (2A10) | MR4 | 2A10 | Mouse | 1:500 |
PfEXP2 | European malaria reagent repository | 7.7 | Mouse | 1:1000 |
PfGAPDH | European malaria reagent repository | 7.2 | Mouse | 1:50000 |
PfMSP1 | Sanaria and NIH/NIAID | AD233 | Mouse | 1:100 |
Human Glutamine Synthetase | Abcam | Ab64613 | Mouse | 1:100 |
Anti-Rabbit Alexa Fluor 594 | Thermofisher Scientific | A11012 | Goat | 1:200 |
Anti-mouse Alexa Fluor 488 | Thermofisher Scientific | A11029 | Goat | 1:200 |
DAPI | Thermofisher | D1306 | NA | 1:300 |
