Fig. 8: Identifying candidate genes’ role in accelerating ESCC progression. | Nature Communications

Fig. 8: Identifying candidate genes’ role in accelerating ESCC progression.

From: Spatial transcriptomics analysis of esophageal squamous precancerous lesions and their progression to esophageal cancer

Fig. 8: Identifying candidate genes’ role in accelerating ESCC progression.The alternative text for this image may have been generated using AI.

a Knockdown and overexpression efficiency of TAGLN2 and CRNN detected by western blot. b Cell proliferation analysis of TAGLN2 silenced or CRNN overexpressed cells by MTT assay (mean ± SD, n = 6 biological replicates). *p = 0.0109, ****p = 3.73e−08, ****p = 3.23e−006 for KYSE150; **p = 0.0015, ****p = 3.09e−005, ****p = 1.22e−005 for KYSE510; ** p = 0.0018, ****p = 3.13e−008, **p = 0.0038 for KYSE450; ***p = 0.0004, ****p = 9.01e−007, ****p = 1.76e−006 for KYSE140 by a two-tailed unpaired t-test at 24 h, 48 h, and 72 h, respectively. c TAGLN2 silenced or CRNN overexpressed cells colony formation ability assessed by anchorage-independent cell growth assay. d Representative images of anchorage-independent cell growth assay (Scale bar, 200 μm). e Colony formation ability was analyzed by clonogenic formation assay. f Representative images of clonogenic formation assay. g Inhibitory effects of organoid growth by knocking down TAGLN2 or overexpressing CRNN. h Representative pictures of organoids after knocking down TAGLN2 or overexpressing CRNN (Scale bar, 100 μm). i, j Tumor volume and tumor weight are decreased by knocking down TAGLN2 or overexpressing CRNN (n = 6 mice). k Pictures of tumors after mice euthanasia at 46 days. Tumor volume and tumor weight are shown as mean values ± SEM by a one-tailed unpaired t-test (p value for shTAGLN2 tumor weight with Welch’s correction). For Tumor volume comparison of shTAGLN2 and shNC: day 4 (*p = 0.0392), day 8 (*p = 0.0379), day 11 (**p = 0.0030), day 15 (**p = 0.0037), day 43 (*p = 0.0330), day 46 (*p = 0.0168). For Tumor volume comparison of CRNN and NC: day 11 (*p = 0.0171), day 22 (*p = 0.0128), day 25 (**p = 0.0021), day 29 (**p = 0.0028), day 32 (***p = 0.0008), day 36 (***p = 0.0005), day 39 (**p = 0.0022), day 43 (**p = 0.0070), day 46 (***p = 0.0009). The data (c, e, g) are shown as percentages compared with the control group by a two-tailed unpaired t-test with Welch’s correction (n = 3, independent experiments, mean ± SD). Source data are provided as a Source Data file.

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