Fig. 2: Construction and characterization of PMZH. | Nature Communications

Fig. 2: Construction and characterization of PMZH.

From: A bimetallic nanoplatform for STING activation and CRISPR/Cas mediated depletion of the methionine transporter in cancer cells restores anti-tumor immune responses

Fig. 2: Construction and characterization of PMZH.

a Schematic structure of PX330 vector after inserting sgRNA and sequencing result of the sgRNA targeting SLC43A2 in CRISPR-Cas9 plasmid. b 1% agarose gel image. Lane 1-3, DNA ladder, naked pDNA, MZ, respectively; lane 4–7, MZ/pDNA at mass ratio of 40:1, 45:1, 50:1, and 55:1, respectively. c TEM image and corresponding element mappings of PMZ. Scale bar is 100 nm. d XRD analysis of PMZ/MZ nanoparticles. e Gel electrophoresis assay of PMZH after incubation in buffers with different pH. Lane 1-2, DNA ladder, naked pDNA, respectively; lane 3–5, PMZH incubation in acid buffers after 3 h, 6 h, 24 h, respectively; lane 6, PMZH incubation in buffers with pH 7.4 after 24 h. f Ion release at various time and under various conditions. n = 3 independent samples. Representative results showed in panel b–e were obtained from three independent samples. Data were presented as mean ± SD. Source data are provided as a Source Data file.

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