Fig. 1: CD83 is expressed in a distinctive regional pattern associated with white matter. | Nature Communications

Fig. 1: CD83 is expressed in a distinctive regional pattern associated with white matter.

From: Microglial expression of CD83 governs cellular activation and restrains neuroinflammation in experimental autoimmune encephalomyelitis

Fig. 1

a Flow cytometric assessment of CD83 expression in CD83eGFP reporter animals under homeostatic conditions. Cells were pre-gated on single living cells, and the percentage of eGFP+ cells was assessed. Wild-type cells served as negative controls. b Summary of the cellular composition among CD45+eGFP+ cells. Data represent the mean of four different animals. c Histograms of eGFP fluorescence in different cell types isolated from the CNS. Wild-type cells served as negative fluorescence controls. Histograms are representative of four individual mice. d Assessment of eGFP signal in microglia from the cortex (Ctx), hippocampus (Hip), cerebellum (Cbm), brainstem (Stem), and spinal cord (SC). Median fluorescence was normalized to cortical microglia (n = 7, and each dot represents pooled tissue from two mice). Data are represented as mean ± SEM. Statistical significance was calculated with one-way ANOVA with Dunnett’s multiple comparison test. e Immunofluorescence of human brain tissue. Microglia are stained for Iba1 (red) and CD83 (green). Nuclear staining was performed with DAPI. Length of all scale bars: 100 µm.

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