Table 1 Primers used for qPCR
Gene | Orientation | Sequence |
|---|---|---|
Apoe | Forward | 5’-TCAGCTCGAGTGGCAAAG-3’ |
Reverse | 5’-TTACTTCCGTCATAGTGTCCTC-3’ | |
Ccl2 | Forward | 5’-GAGAGCTACAAGAGGATCACC-3’ |
Reverse | 5’-GATCTCATTTGGTTCCGATCC-3’ | |
Ccl3 | Forward | 5’-TCTGTCACCTGCTCAACATC-3’ |
Reverse | 5’-CGATGAATTGGCGTGGAATC-3’ | |
Ccl4 | Forward | 5’-GCTGTTTCTCTTACACCTCCC-3’ |
Reverse | 5’-GTTCAACTCCAAGTCACTCATG-3’ | |
Ccl5 | Forward | 5’-CCCACGTCAAGGAGTATTTCT-3’ |
Reverse | 5’-ACCCTCTATCCTAGCTCATCTC-3’ | |
Cd83 | Forward | 5’-CGCAGCTCTCCTATGCAGTG-3’ |
Reverse | 5’-GTGTTTTGGATCGTCAGGGAATA-3’ | |
Clec7a | Forward | 5’-GAATCCTGTGCTTTGTGGTAG-3’ |
Reverse | 5’-GGAGCCACCTTCTCATCTAAAG-3’ | |
Entpd1 | Forward | 5’-ACCGAACTGATACCAACATCC-3’ |
Reverse | 5’-CCTTCCTCTTGTCCAGTGATG-3’ | |
Ifng | Forward | 5’-GCTTTGCAGCTCTTCCTCAT-3’ |
Reverse | 5’-GTCACCATCCTTTTGCCAGT-3’ | |
Il6 | Forward | 5’-ACAAAGCCAGAGTCCTTCAGAG-3’ |
Reverse | 5’-GAGCATTGGAAATTGGGGTAGG-3’ | |
Il12a | Forward | 5’-AACAGGGTGATGGGCTATC-3’ |
Reverse | 5’-TGAGATGTGATGGGAGAACAG-3’ | |
Il23a | Forward | 5’-GCAACTTCACACCTCCCTAC-3’ |
Reverse | 5’-CGAAGGATCTTGGAACGGAG-3’ | |
Lpl | Forward | 5’-TAGACAACGTCCACCTCTTAG-3’ |
Reverse | 5’-ACATCTACAAAATCAGCGTCATC-3’ | |
Mmp9 | Forward | 5’-GCTGACTACGATAAGGACGGCA-3’ |
Reverse | 5’-TAGTGGTGCAGGCAGAGTAGGA-3’ | |
Msr1 | Forward | 5’-AGGTGTTAAAGGTGATCGGG-3’ |
Reverse | 5’-ATCTTGATCCGCCTACACTC-3’ | |
Rpl4 | Forward | 5’-GCTGAACCCTTACGCCAAGA-3’ |
Reverse | 5’-TCTCGGATTTGGTTGCCAGT-3’ | |
Siglech | Forward | 5’-GCAAGAGATCCCATACGAAGAG-3’ |
Reverse | 5’-AGTCCAGTTGGCACCATC-3’ | |
Spp1 | Forward | 5’-ACTTTCACTCCAATCGTCCC-3’ |
Reverse | 5’-GTCCTCATCTGTGGCATCAG-3’ | |
Tbx21 | Forward | 5’-AGCAAGGACGGCGAATGTT-3’ |
Reverse | 5’-GGGTGGACATATAAGCGGTTC-3’ | |
Tmem119 | Forward | 5’-GTGTCTAACAGGCCCCAGAA-3’ |
Reverse | 5’-AGCCACGTGGTATCAAGGAG-3’ | |
Tnf | Forward | 5’-GTGATCGGTCCCCAAAGGG-3’ |
Reverse | 5’-CCAGCTGCTCCTCCACTTG-3’ | |
Trem2 | Forward | 5’-GGAACCGTCACCATCACTC-3’ |
Reverse | 5’-CCAGCATCTTGGTCATCTAGAG-3’ | |
CD83 (human) | Forward | 5’-TGCTGCTGGCTCTGGTTATT-3’ |
Reverse | 5’-TGTGAGGAGTCACTAGCCCT-3’ | |
RPLP0 (human) | Forward | 5’GCCATTGCCCCATGTGAAGT3’ |
Reverse | 5’AGCTGCACATCACTCAGGATT3’ |
