Fig. 1: Simple model of the initial stages of assembly of PSII and the various isolated RCII complexes. | Nature Communications

Fig. 1: Simple model of the initial stages of assembly of PSII and the various isolated RCII complexes.

From: The Ycf48 accessory factor occupies the site of the oxygen-evolving manganese cluster during photosystem II biogenesis

Fig. 1

The D1 module contains precursor D1 (pD1), PsbI (I) and Ycf48 (Y48) and the D2 module contains D2, the PsbE (E) and PsbF (F) subunits of Cyt b-559 and CyanoP (P). Precursor D1 is cleaved to intermediate D1 (iD1) and mature D1 upon formation of the RCII complexes, RCIIa and RCII*. RCII* is equivalent to RCIIa but additionally contains the Ycf39 (Y39)/Hlips (High light inducible proteins) complex. The Hlips complex (HliCD) consists of the HliC and HliD subunits. The rubredoxin-like RubA protein is found in RCIIa and RCII*. The His-tagged RCII/PSI complex studied here was purified by immobilised Ni2+-affinity chromatography followed by sucrose density gradient centrifugation. Only the large PsaA and PsaB RC subunits are displayed in PSI for clarity.

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