Fig. 1: Physiological responses of mature soybean nodules to different N treatments in hydroponic culture. | Nature Communications

Fig. 1: Physiological responses of mature soybean nodules to different N treatments in hydroponic culture.

From: The NAC transcription factors SNAP1/2/3/4 are central regulators mediating high nitrogen responses in mature nodules of soybean

Fig. 1

a A hydroponic culture system for reversible N treatment. INC inoculation, DAI day after inoculation, HN high N treatment, LN low N treatment; numbers indicate the days after treatment. b ARA activity of mature nodules under different N treatments. ARA acetylene reduction assay, DAT day after treatment. Data are represented as mean ± SD (n = 8). c Cross section observations of mature nodules under different N treatments. Scale bar = 1 mm. More sections were shown in Supplementary Fig. 1a. d Relative leghemoglobin change of nodule samples under different N treatments. Leghemoglobin content of nodule samples under different N treatments were shown in Supplementary Fig. 2a. Data are represented as mean ± SD (n = 12). e SYTO9 staining for rhizobia in mature nodules under different N treatments. More sections of SYTO9 staining were shown in Supplementary Fig. 3a. Scale bar = 50 μm. f Relative fluorescence intensity of SYTO9 staining for rhizobia in mature nodules under different N treatments. Fluorescence intensity of SYTO9 staining for rhizobia in mature nodules under different N treatments were shown in Supplementary Fig. 2b. Data are represented as mean ± SD (n = 20). g Transmission electron micrographs of mature nodules under different N treatments. More micrographs were shown in Supplementary Fig. 5b. Up panel: Scale bar = 10 μm; down panel: Scale bar = 1 μm. V lytic vacuolar compartments. PHB was indicated by red arrow. Source data underlying b, d, and f are provided as a Source Data file.

Back to article page