Fig. 1: Identification of three distinct states in drug-free TMEM16F.
a Gaussian filtered density of the nanodisc and unsharpened density of the protein dimer. Unsharpened cryo-EM density and atomic model for three states with different conformations of TM6. Right, Ca2+ binding sites, with sharpened cryo-EM density in semitransparent outline and the residues depicted as sticks colored by heteroatom, in (b) monomer with extended TM6 and (c) monomer with kinked TM6. d Electrostatic surface of the asymmetric TMEM16F dimer, where white represents hydrophobic areas and blue and red correspond to positively and negatively charged regions, respectively.
