Fig. 7: Cryo-EM structures of ICs with m6A in different positions of the A-site codon.
From: Modulation of translational decoding by m6A modification of mRNA
a From top to bottom: AAA, m6AAA, Am6AA, AAm6A. Left panel: schematic view; right panel: map colored by the RNA chain. LSU is shown in blue, SSU in yellow, mRNA in red, P-site tRNA in cyan. The A-site codon is indicated with dashed circles. b Structures of unreacted ICs (i.e. prepared without addition of Lys-tRNALys, see text) with AAA mRNA (left) and Am6AA mRNA (right). Maps are shown in surface representation. c Close-up view of interactions between AAA or AAm6A A-site codon (red), and rRNA (yellow) in the decoding region of the unreacted ICs (top). The corresponding models are also shown from different perspectives (bottom). In the unreacted AAA (left) and AAm6A ICs (middle) the nucleotides form a π-stacking array, whereas the equivalent nucleotides in the post-decoding complex (P/P A/A) appear unstacked (right). d Repositioning of the 16S rRNA nucleotides A1493, A1492, G530 and C1397. The conformational rearrangements during the transition from IC (yellow) to the post-decoding complex (P/P A/A; gray) are shown in reference to the mRNA codon.
