Fig. 5: Expression of transcription factors in single EVT cells derived from human placenta. | Nature Communications

Fig. 5: Expression of transcription factors in single EVT cells derived from human placenta.

From: Extravillous trophoblast cell lineage development is associated with active remodeling of the chromatin landscape

Fig. 5

Data from two publicly available, independent, single-cell RNA sequencing (scRNA-Seq) datasets from first trimester placentas27,28 are used and cell types are inferred using marker genes published previously28 as decidual macrophages (dM), fetal (Endo f) and maternal endothelial cells (Endo m), epithelial glandular cells (Epi), extravillous trophoblast cells (EVT), fetal fibroblasts (FB), Hofbauer cells (HB), syncytiotrophoblast and villous cytotrophoblast (SCT/VCT) and cells not determined (ND). Single cell expression values (normalized, scaled and natural-log transformed) for five transcription factors (ASCL2; DLX6; EPAS1, MYCN and SNAI1) shown in (a) Violin plots (shown as median and 25th and 75th percentiles; points are displayed as outliers if they are above or below 1.5 times the interquartile range) comparing EVT cells (orange, N = 693 cells) with syncytiotrophoblast (SCT) and villous cytotrophoblast cells (VCT) combined (turquoise, N = 7834 cells) and (b) UMAP feature plots (red color scale of expression values thresholded at the 5th and 95th percentiles).

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