Fig. 2: MVA-ID vaccinated animals show different correlates of protection compared to MVA-IM vaccinated animals. | Nature Communications

Fig. 2: MVA-ID vaccinated animals show different correlates of protection compared to MVA-IM vaccinated animals.

From: Intradermal but not intramuscular modified vaccinia Ankara immunizations protect against intravaginal tier2 simian-human immunodeficiency virus challenges in female macaques

Fig. 2

a BG505_SOSIP-specific IgG in serum. Statistical significance by Mann–Whitney rank-sum (two-tailed) test. b, c BG505_SOSIP-specific IgG and IgA in vaginal secretions; Vaginal IgG: (MVA-ID: n = 9 at MVA-1 and n = 10 at rest of the time points; MVA-IM: n = 10 at all time points); Vaginal IgA: (MVA-ID: n = 9 at MVA-1 and Prt-2 time ponts and n = 10 at rest of the time points; MVA-IM: n = 10 at all time points). Statistical significance by Mann–Whitney rank-sum (two-tailed) test. d Neutralization antibody ID50 titers against BG505.T332N Env pseudovirus in serum. e Spearman’s correlation (two-sided) between neutralizing antibody titers and rate of virus acquisition. The dotted line represents the neutralization titer at 50. f, g Kaplan–Meier survival curves in MVA-ID and MVA-IM groups with f high neutralizing antibody titer and g low-neutralizing antibody titer, compared to control animals. Statistical significance by log-rank test. h Serum antibody effector functions compared between MVA-ID and MVA-IM groups. Left to right: ADCVI inhibition, ADCC activity, ADP score. i Vaginal IgA and j serum ADP score in protected and infected animals within a group and compared between both vaccinated groups. Statistical significance by Mann–Whitney rank-sum (two-tailed) test; n = 4 in MVA-ID protected; n = 6 in MVA-ID infected; n = 3 in MVA-IM protected; n = 7 MVA-IM infected. Spearman’s correlation (two-sided) between rate of virus acquisition and k) anti-BG505 SOSIP vaginal IgA, l serum ADP score. In all the correlation plots, p and r values are color-coded to represent the vaccine group (red: MVA-ID, Blue: MVA-IM, Black: both groups combined). These values were not shown for the group that did not have a statistically significant p value <0.05. Boxes in all the box plots extend from the 25th percentile to the 75th percentile of the dataset and the line inside the box denotes the median. The whiskers outside the box descend to the dataset’s minimum values and ascend to the maximum values. MVA-1: first MVA; MVA-2: second MVA; Prt-1: first protein; Prt-2: second protein; Prt-3: third protein, UI uninfected. Source data are provided as a Source Data file.

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