Fig. 6: Physiochemical features of TSPAN2-, TSPAN3- and CD63-enriched EVs.
From: Identification of scaffold proteins for improved endogenous engineering of extracellular vesicles
a Size distribution of EVs from transfected HEK-293T cells. b Representative cryo-electron microscopy images of EVs. c Western blots of positive and negative markers of EVs. d Cellular location of tetraspanins in transfected HEK-293T cells. e Single-vesicle flow cytometry dot plots of EVs after staining with APC-conjugated CD9/CD63/CD81 tetraspanin antibodies. f Volcano plots showing differentially enriched proteins in EVs. The red digits indicate the count of differentially enriched proteins. Results were from three biological replicates. Source data are provided as a Source Data file. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD043840.
