Fig. 7: N1 neutrophils and their chemoattractants (CXCL1, CXCL2, and CXCL5) are abundant in the synthetic MNs and the endogenous lungs of C57 mice with EO771 weakly metastatic breast cancer. | Nature Communications

Fig. 7: N1 neutrophils and their chemoattractants (CXCL1, CXCL2, and CXCL5) are abundant in the synthetic MNs and the endogenous lungs of C57 mice with EO771 weakly metastatic breast cancer.

From: A synthetic metastatic niche reveals antitumor neutrophils drive breast cancer metastatic dormancy in the lungs

Fig. 7

a EO771-GFP tumor cells migrated from the primary site to the diseased scaffolds and lungs but failed to form macroscopic metastases in C57 mice. The humane endpoint of C57 mice bearing EO771-GFP tumor without treatment was ~40 days after orthotopic tumor inoculation due to the large size of the primary tumor. b, c The frequencies of Gr1+CD11b+Ly6G+ neutrophils (b) and the expression of S100a8 and S100a9 genes (c) in diseased scaffolds and lungs of C57 mice bearing EO771-GFP tumor compared to healthy tissues of tumor-free C57 mice. d The frequencies of immune cells with pro-inflammatory and antitumor phenotypes in scaffolds and lungs derived from mice bearing EO771-GFP tumor. e The proteins secreted by diseased lungs of C57 mice bearing EO771 tumor compared to those secreted by diseased lungs of BALB/c mice bearing 67NR, 4T07, or 4T1 tumor or those secreted by healthy lungs of tumor-free C57 mice. f The proteins secreted by healthy lungs of tumor-free C57 mice compared to those secreted by healthy lungs of tumor-free BALB/c mice. g Size of the EO771-GFP primary tumor in wild type (WT), Ifng−/−, and Il17a/Il17f−/− C57 mice at day 28 after orthotopic tumor inoculation. h, i The abundance of CXCL1, CXCL2, and CXCL5 proteins (h) and the frequencies of immune cells with pro-inflammatory and antitumor phenotypes (i) in the diseased lungs of wild type (WT), Ifng−, and Il17a/Il17f−/− C57 mice bearing EO771-GFP tumor. j Schematic illustration of the selective recruitment of N1 and N2 neutrophils to the lungs of animal models of breast cancers with varying tumor aggressiveness responding to distinct groups of neutrophil chemoattractants, followed by creating an immunosuppressive or dormant environment in the lungs. Data are shown as Mean ± SEM and p values are from student’s two-tailed t test. n = 6 for (a), n = 3 for (b), (c), (d), (i), and n = 4 for (g). Source data are provided as a Source Data file.

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