Fig. 3: Dynamics within the RodA.
From: Structural basis of peptidoglycan synthesis by E. coli RodA-PBP2 complex
a RodA shown as ribbon colored by domain with TM helices 3-7 red and TM helices 1-2 and TM helices 8-10 blue. b Local resolution of the transmembrane region of the RodA-PBP2 complex from the final local resolution refinement with a mask around the transmembrane part of the complex. It is apparent that the domain consisting of TM helices 3-7 overall has lower local resolution. c Sterically allowed rotamers of nitroxide spin labels (R1) are illustrated for Gly44R1/Asp90R1 on the cryo-EM structure (orange sidechains). d Resulting DEER distance distributions (solid black line) are compared to predicted distance distributions (bars) based on the sterically allowed rotamers shown in the structures (colored as sidechains).
