Fig. 1: Genome-wide excess H3K9me3 modification throughout SCNT preimplantation development. | Nature Communications

Fig. 1: Genome-wide excess H3K9me3 modification throughout SCNT preimplantation development.

From: Unreprogrammed H3K9me3 prevents minor zygotic genome activation and lineage commitment in SCNT embryos

Fig. 1: Genome-wide excess H3K9me3 modification throughout SCNT preimplantation development.The alternative text for this image may have been generated using AI.

a Schematic of the preparation of mouse somatic cell nuclear transfer (SCNT) embryos for genome-wide ChIP-seq analysis of H3K9me3. Samples of donor cells (cumulus cells, CC) and 6 h post activation (hpa), 14 hpa, 2-cell (2C), 4-cell (4C), 8-cell (8C) and morula-stage embryos and the inner cell mass (ICM) and trophectoderm (TE) of E4 blastocysts were analyzed. b Genome Browser view of RPKM value of H3K9me3 signals around the Zscan4d, Dux and MERVL loci in SCNT (orange) and fertilized (cyan) embryos. c The percentage of genomic regions covered by H3K9me3 peaks in SCNT and fertilized embryos. d The percentage of H3K9me3 peaks in SCNT embryos overlapping with the corresponding stage of fertilized embryos. e The fractions of established and removed H3K9me3 domains during SCNT embryo development. f The percentage of H3K9me3 in SCNT and fertilized embryos overlapping with that in donor cells (CC) during preimplantation development. Source data are provided as a Source Data file.

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