Fig. 6: Overexpression of Max or Mcrs1 significantly improves cumulus cell-derived SCNT development.
a Schematic of the strategy for overexpression of Max and Mcrs1 in SCNT embryos. Control (H2O), Max (50–100 ng/µL) and Mcrs1 (250–500 ng/µL) mRNA were injected into both blastomeres of late 2-cell embryos. b Representative images of Max and Mcrs1 OE embryos at E4. Scale bar, 100 μm. c Overexpression of Max or Mcrs1 significantly improved the blastocyst rate of SCNT embryos. Control, 226 embryos, r = 7; Max, 240 embryos, r = 6; Mcrs1, 171 embryos, r = 5. r indicates the number of replicates. d Representative images of E10.5 Mcrs1OE SCNT embryos. One representative result of at least three independent experiments is shown. Scale bar, 2 mm. e Mcrs1 OE greatly improved the implantation ability of SCNT embryos. N indicates the number of transferred 2-cell embryos. R indicates the number of transferred pseudopregnant female mice. Spots connected by a dotted line represent the same pseudopregnant female mouse. f The birth rate of full-term SCNT pups after Mcrs1 OE. N indicates the number of transferred 2-cell embryos. R indicates the number of transferred pseudopregnant female mice. g Unrooted dendrogram of H3K9me3 signals between the ICM and TE from fertilized, SCNT and SCNT+Mcrs1 embryos mediated from hierarchical cluster analysis. h H3K9me3 signals in fertilized, SCNT and SCNT+Mcrs1 blastocysts on the binding sites of MCRS1. i H3K9me3 signals in ICM and TE of fertilized, SCNT and SCNT+Mcrs1 embryos, and MCRS1 binding signals (published data from GSE51746) around the Nckap1, Mfsd1, and Cdv3 loci (c, f). Data are presented as mean values ± SEM (c, e, f). Statistical significance was calculated with a two-tailed Student’s t test (h). Statistical significance was calculated with a two-tailed Wilcoxon rank-sum test. Source data are provided as a Source Data file.
