Fig. 1: Nanoclusters of Neurexins and PtprS are localized in the same inhibitory synapses of deep cerebellar nuclei in mice. | Nature Communications

Fig. 1: Nanoclusters of Neurexins and PtprS are localized in the same inhibitory synapses of deep cerebellar nuclei in mice.

From: Combinatorial expression of neurexins and LAR-type phosphotyrosine phosphatase receptors instructs assembly of a cerebellar circuit

Fig. 1

a Schematic of selected trans-synaptic adhesion complexes formed by Neurexins and LAR-PTPRs. b, c, Profiling of Neurexin and LAR-PTPR transcripts in cerebellar Purkinje cells. Experimental design (b) and quantification (c; means ± SEM; n = 4 mice (2 females and 2 males)) of ribosome-bound mRNAs (IP) from the cerebellum of RiboTag mice crossed to L7-cre mice (which express Cre only in Purkinje cells) followed by quantitative RT-PCR. Data were normalized to actin and compared to total mRNA (input). PtprF mRNA was not detected in the ribosome-bound fraction. Calb1 and Pcp were used as specific markers for Purkinje cells. d–g Localization of both Neurexins and PTPRS in most synapses of deep cerebellar nuclei (DCN) that receive inputs from Purkinje cells. d Experimental design for e–g. e Representative images for Neurexin (green, 647) and PTPR clusters (magenta, 647) visualized using direct stochastic optical reconstruction microscopy (dSTORM); Purkinje cell boutons in the DCN are visualized with antibodies to vGAT (blue, 568); f, pie charts showing the distribution of Purkinje cells boutons without (0) or with Neurexins (green) or PTPRS (magenta) clusters (1, 2, and 3+); g, violin plots illustrating the properties of Neurexins (green), PTPRS (magenta) and vGAT (blue) nanoclusters as analyzed by dSTORM; n = 213 (5 ROIs/ 1 mouse) clusters for Nrxn, n = 204 (5 ROIs/ 1 mouse) clusters for PTPRS, n = 317 (10 ROIs/ 1 mouse) clusters for vGAT). Source data are provided within the Source Data file.

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