Fig. 1: Auranofin (AF) binds to UBA1 and enhances UBA1 interaction with UBE2G2. | Nature Communications

Fig. 1: Auranofin (AF) binds to UBA1 and enhances UBA1 interaction with UBE2G2.

From: Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes

Fig. 1: Auranofin (AF) binds to UBA1 and enhances UBA1 interaction with UBE2G2.The alternative text for this image may have been generated using AI.

a Chemical structure of AF. b, c AF enhances UBA1 interaction with UBE2G2 in cells. Total lysates from 293 T cells treated with 100 nM AF for 3 h were used for anti-UBA1 (a) or anti-UBE2G2 (b) co-immunoprecipitation (coIP) assays. Lane 3 is anti-IgG control. d Effects of AF on the thermal stability of UBA1 and AUP1 in UBE2G2 knockout 293 T cells as revealed by CETSA. The indicated proteins were blotted in soluble fractions. e, f The intensities of the bands for UBA1 and AUP1 in (d) were quantified. Data are presented as mean values ± S.D., n = 3 biologically independent experiments. p-value was calculated by two-tailed paired t-test. *p < 0.05 and **p < 0.01 for UBA1. p = 0.008, p = 0.0421 and p = 0.0285 at 47, 52 and 57 oC, respectively. No difference for AUP1. Source data are provided as a Source Data file.

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