Fig. 7: AF does not enhance NHK ubiquitination and proteasomal degradation in HCT116 cells expressing C1039A mutant UBA1 (HCT116-UBA1(C1039A) cells). | Nature Communications

Fig. 7: AF does not enhance NHK ubiquitination and proteasomal degradation in HCT116 cells expressing C1039A mutant UBA1 (HCT116-UBA1(C1039A) cells).

From: Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes

Fig. 7: AF does not enhance NHK ubiquitination and proteasomal degradation in HCT116 cells expressing C1039A mutant UBA1 (HCT116-UBA1(C1039A) cells).The alt text for this image may have been generated using AI.

a AF does not downregulate NHK in HCT116-UBA1(C1039A). HCT116-UBA1(C1039A) cells were generated by CRISPR/cas9. NHK-HA were transiently expressed in parental HCT116 and two different clones of HCT116-UBA1(C1039A) cells. Cells were treated with AF for 24 h and then processed for IB. b AF does not enhance gp78c-mediated ubiquitination in vitro in presence of UBA1(C1039A). c AF does not increase UBA1-UBE2L3 interaction in HCT116-UBA1(C1039A) cells as revealed by anti-UBA1 coIP. d AF does not promote NHK-HA ubiquitination in HCT116-UBA1(C1039A) cells as revealed by anti-HA reIP as described in Fig. 5f. Source data are provided as a Source Data file.

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