Table 1 AHPO-HPLC data for total melanin marker concentrations (ng/mg) in black, rufous and white feathers
Feather type | HCl pretreatment | Total melanin | Eumelanin | Phaeomelanin | |
|---|---|---|---|---|---|
Black | Untreated | no | 2377 ± 309 | 2173 ± 315 | 204 ± 29 |
yes | 1059 ± 170 | 988 ± 143 | 71 ± 28 | ||
200 °C | no | 2408 ± 474 | 2146 ± 410 | 261 ± 89 | |
250 °C | no | 1144 ± 249 | 819 ± 215 | 325 ± 53 | |
yes | 786 ± 204 | 702 ± 179 | 83 ± 28 | ||
Rufous | Untreated | no | 7984 ± 1411 | 308 ± 67 | 7676 ± 1356 |
yes | 583 ± 60 | 84 ± 16 | 499 ± 54 | ||
200 °C | no | 5178 ± 1041 | 258 ± 54 | 4920 ± 1005 | |
250 °C | no | 2412 ± 379 | 235 ± 40 | 2177 ± 381 | |
yes | 1263 ± 91 | 195 ± 29 | 1068 ± 68 | ||
White | Untreated | no | 43 ± 7 | 20 ± 2 | 24 ± 5 |
yes | 21 ± 4 | 8 ± 0 | 13 ± 4 | ||
200 °C | no | 51 ± 13 | 22 ± 4 | 29 ± 10 | |
250 °C | no | 226 ± 19 | 85 ± 31 | 141 ± 48 | |
yes | 173 ± 36 | 152 ± 39 | 22 ± 4 | ||
Percentage Change In Marker Concentration Relative To Untreated Samples | |||||
Black | 200 °C | no | +1 | −1 | +28 |
250 °C | no | −52 | −62 | +59 | |
yes | −26 | −28 | +18 | ||
Rufous | 200 °C | no | −35 | −16 | −36 |
250 °C | no | −69 | −24 | −72 | |
yes | +116 | +132 | +114 | ||
White | 200 °C | no | +18 | +12 | +22 |
250 °C | no | +420 | +333 | +491 | |
yes | +712 | +1807 | +63 | ||