Fig. 6: Exogenous VHL expression suppresses white adipose tissue lipolysis and browning.

AAV-VHL viruses or AAV-GFP viruses were injected into iWAT and eWAT of both sides 1 week after implantation of 4T1 cells. Organs were collected on day 42 after implantation. All the parameters were collected from AAV-VHL and AAV-GFP mice. a Body weight (n = 5 mice per group; unpaired two-tailed t-test). b Relative fat tissues weight (normalized to body weight) (n = 5 mice per group; unpaired two-tailed t-test). c Daily food intake (n = 5 mice examined by 10 independent experiments per group; unpaired two-tailed t-test). d Rectal temperatures of the mice at different times after cold exposure (n = 3 mice per group; unpaired two-tailed t-test). e Oxygen consumption (VO₂) and f Heat production. Dot plots represent the 48-hour average values (n = 5 mice per group; unpaired two-tailed t-test). g Relative mRNA abundance of the indicated thermogenic markers in BAT from each group mice (n = 5 mice per group; unpaired two-tailed t-test). h Relative mRNA abundance of the indicated mRNA levels in iWAT and eWAT from each group mice (n = 4 mice per group; unpaired two-tailed t-test). i Western blots showing indicated proteins in iWAT and eWAT from each group mice (tumour free group, n = 4 mice per group; TB AAV-GFP group, n = 4 mice per group; TB AAV-VHL group, n = 4 mice per group). j The serum leptin levels (n = 5 mice per group) and k level of norepinephrine (NE) in iWAT and eWAT (n = 5 mice per group), unpaired two-tailed t-test. l Plasma-free fatty acid levels in the two group (n = 5 mice per group; unpaired two-tailed t-test). m Representative H&E staining with iWAT/eWAT from each group mice. Repeated three times independently with similar results obtained. Scale bar, 200  μm. n Representative immunohistochemistry staining of HIF1Α in iWAT and eWAT. Repeated three times independently with similar results obtained. Scale bar, 200 μm. o Representative immunohistochemistry staining of UCP1 in iWAT and eWAT. Repeated three times independently with similar results obtained. Scale bar, 200 μm. Data are presented as mean ± s.e.m; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001, ns: not significant. Source data and exact P value are provided as a Source data file.