Fig. 5: MTH1 deficiency reduces the expression of mtDNA-encoded genes after thrombin stimulation.
a Western blot analysis of the expression of MUTYH, OGG1/2, MTH2, and MTH3 in MTH1fl/fl and MTH1-/- platelets under resting condition. The representative images were shown from three independent experiments (mean ± SD, n = 3 independent isolated platelets). b Mice were administered a Dylight 488-labeled anti-GP1bβ antibody (Emfret, X488) via tail vein (0.1 μg/g body weight) to measure platelet half-life by flow cytometry. Data were presented as mean ± SE (n = 3, two-way ANOVA with Sidak multiple comparisons test. c Expression of subunit of complexes (CI, CIII, CIV and CV) in the mitochondrial respiration chain proteins encoded by mtDNA (ND1, CYTB, MT-CO1, ATP8) or nucleus DNA (NDUFV1, UQCRC2, COX6A1, ATP5A) in platelets from MTH1fl/fl or MTH1−/− mice before (−) and after (+) stimulation with thrombin (1 U/ml) for 3 mins (representative of three independent experiments) (mean ± SD, n = 3, two-way ANOVA with Sidak multiple comparisons test). d MT-CO1 gene expression level in thrombin-activated platelets from MTH1fl/fl or MTH1−/− mice was measured by quantitative real-time PCR and represented as a fold change relative to its level in resting platelets (mean ± SD, n = 3 independent isolated platelets, two-tailed unpaired Student’s t test). e Analysis of the number of guanine in the 13 mtDNA sequences.
