Fig. 2: Fluorescence reporter system for differentiating myofibroblasts activated by profibrotic alveolar organoids. | Nature Communications

Fig. 2: Fluorescence reporter system for differentiating myofibroblasts activated by profibrotic alveolar organoids.

From: Autocrine TGF-β-positive feedback in profibrotic AT2-lineage cells plays a crucial role in non-inflammatory lung fibrogenesis

Fig. 2

a Diagram of co-culture using AOs from wild-type (WT) mice with primary lung fibroblasts from Acta2-DsRed mice. b Representative images of co-cultured (myo)fibroblasts (scale bar: 100 μm) and quantification of the number and the relative size of DsRed+ myofibroblasts and the relative number of total fibroblasts around the AO-containing gel. Data represent the mean ± SEM of results obtained from three independent mice. *P < 0.05, **P < 0.01 (unpaired, two-tailed Student’s t test). c Diagram of sensitivity analysis using Acta2-DsRed lung fibroblasts using mixed TGF-β1/β2/β3. d Representative images of (myo)fibroblasts (scale bar: 1 mm) and quantification of the relative DsRed+ area around the gel. Data represent the mean ± SEM of results obtained from three independent mice. **P < 0.01, ***P < 0.001 (one-way analysis of variance with Bonferroni correction).

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