Fig. 2: Transiently inducing PT elevation in early-adulthood abolishes longevity benefits of dwarf flies.
From: Early-adulthood spike in protein translation drives aging via juvenile hormone/germline signaling
a Early-adulthood PT in daGS > UAS-S6KKQ flies after ± 200 µM RU486 (day 0–10), determined by puromycin incorporation normalized to Ponceau staining. n = 3/group; two-way ANOVA with Sidak post-hoc test. b Experimental scheme to transiently manipulate PT in different life stages; 200 µM RU486 given to daughterless-GeneSwitch GAL4 (daGS)>UAS-S6KKQ flies during early-adulthood (day 0–10), late-adulthood (day 40–50), or whole-adulthood. c In daGS > UAS-S6KKQ flies, early-adulthood (day 0–10) RU486 prolongs lifespan just like whole-adulthood RU486. Late-adulthood (day 40–50) RU486 does not alter lifespan. Each sex: n = 250/group; log-rank test. Puromycin incorporation in (d), chico homozygotes (female) and (e), chico heterozygotes (female) vs. wild-types. Absence of early-adulthood PT elevation in chico homozygotes and chico heterozygotes. n = 3/group; two-way ANOVA with Sidak post-hoc test. Male data in Supplementary Fig. 1. f Experimental scheme to induce the early-adulthood PT elevation in chico homozygotes; ± 200 µM RU486 given to chico1/chico1, UAS-S6KTE; tubulin-GeneSwitch (tubGS) GAL4 flies during early-adulthood (day 0–4). g Puromycin incorporation in chico1/chico1, UAS-S6KTE; tubGS GAL4 flies (±RU486, day 0–4). n = 3/group; two-way ANOVA with Sidak post-hoc test. h Early-adulthood S6KTE overexpression shortens lifespan and largely abolishes longevity of chico homozygotes. chico flies without early-adulthood S6KTE inductions vs. controls. Female: + 34.4%, male: + 37.7% (% change in median lifespan); chico flies with early-adulthood S6KTE inductions vs. controls. Female: + 6.3%, male: + 8.2%. Early-adulthood S6KTE overexpression does not significantly alter lifespan of +/+ controls. Each sex: n = 250/group; log-rank test. Data shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file.
