Fig. 5: B cells express early activation genes in a poised state.
a, d, g mRNA abundance in TPM of selected early activation genes as measured by Illumina sequencing (n = 4 mice; mean ± SD). Genome browser view displays individual long-reads from ONT sequencing of Cd69 (chr6: 129,266,982-129,275,447) (b), Nr4a1 (chr15:101,254,093-101,275,115) (e), Myc (chr15: 61,983,341-61,992,361) (h), visualized with the Integrative Genomics Viewer. RefSeq GRCm38 annotation (blue), coverage of (dark grey) and aligned long-reads (light grey) were reported for each gene. Lines connecting light grey boxes indicate splicing junctions between aligned sequences. In squared brackets the maximum read coverage for each B cell population. c, f, i Black symbols show protein detection of indicated genes within our proteomic dataset. Grey boxes represent protein copy numbers (mean ± SD) for the 100 protein-coding genes with closest RNA expression (log2 TPM) to the indicated gene. The centre and bounds of the boxes represent the median and interquartile range, respectively. The upper and lower whiskers extend to the maximum and minimum values, respectively, or, in cases where outliers are displayed, to the 75th/25th percentile +/− 1.5x interquartile range. Source data are provided as Source Data file.
